NSF Bread Grant

cassava

National Science Foundation (NSF) Basic Research to Enable Agricultural Development (BREAD): Molecular and Genomic Strategies to Engineer Durable and Sustainable Disease Resistance to Bacterial Blight of Cassava


Cassava bacterial blight, incited by Xanthomonas axonopodis pv. manihotis (Xam) is a serious threat to cassava plants in several developing countries in both Africa and South America. We have sequenced the genomes of 65 Xam strains. We used the sequence information to annotate and characterize the constellation of bacterial effector (virulence) proteins that are present in naturally occurring field isolates of this important bacterial pathogen. This information can be employed to identify new sources of disease resistance in wild populations of cassava plants. To read more about this work, to find out how the Xam genomes were assembled and annotated, and for information on the strains analyzed, please read the following publication:

Bart, R., Cohn, M., Kassen, A., McCallum, E. J., Shybut, M., Petriello, A., Krasileva, K., Dahlbeck, D., Medina, C., Alicai, T., Kumar, L., Moreira, L. M., Neto, J. R., Verdier, V., Santana, M. A., Kositcharoenkul, N., Vanderschuren, H., Gruissem, W., Bernal, A., and Staskawicz, B. J. 2013. Correction for Bart et al., High-throughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance. Proc. Natl. Acad. Sci. U. S. A. 110:19969          http://www.pnas.org/content/109/28/E1972.long

Resources:

Python scripts to identify conserved effectors can be accessed at the following link: https://pypi.python.org/pypi/biotools/1.1.2

Analyzed Xam strains are available at the French collection of plant-associated bacteria (CFBP): http://www-intranet.angers.inra.fr/cfbp/index_e.html, and at the Brazilian Biological Institute Culture Collection of Phytopathogenic Bacteria (IBSBF):http://www.biologico.sp.gov.br/bacterias.php

A list of sequenced Xam strains be found here: https://docs.google.com/spreadsheets/d/1kiZa7mvj73tpppF8I7Cx3JaGfdAE6sojq91QedyIXwg/pubhtml